type strains s aureus atcc 25923 mssa  (ATCC)

Journal: International Journal of Molecular Sciences

Article Title: Nusbiarylins Inhibit Transcription and Target Virulence Factors in Bacterial Pathogen Staphylococcus aureus

doi: 10.3390/ijms21165772

Figure Lengend Snippet: Effects of MC4 on the ( i ) time–kill kinetics and ( ii ) ATP production of ( A ) S. aureus type strain ATCC ® 25923 and ( B ) CA-MRSA strain USA300 when challenged at ¼ ×, 1 ×, 4 × and 16 × MIC in TSB media. Experiments were repeated in triplicates. 25923: S. aureus ATCC ® 25923; USA300: CA-MRSA strain USA300.

Article Snippet: Vancomycin had no apparent effect on α-toxin production compared to untreated controls in both S. aureus ATCC ® 25923 type strain and USA300.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Nusbiarylins Inhibit Transcription and Target Virulence Factors in Bacterial Pathogen Staphylococcus aureus

doi: 10.3390/ijms21165772

Figure Lengend Snippet: Western blot analysis of the effects of selected nusbiarylins in the expression of ( A ) α-toxin and ( B ) Panton–Valentine Leukocidin (PVL) in S. aureus ATCC ® 25923; ( C ) α-toxin and ( D ) PVL in MRSA USA300 at subinhibitory concentration, presented as ( i ) blot image and ( ii ) relative intensity. Each experiment was performed in triplicates and representative figures shown. 25923: S. aureus ATCC ® 25923; USA300: CA-MRSA strain USA300; Van: vancomycin; Rif: rifampicin.

Article Snippet: Vancomycin had no apparent effect on α-toxin production compared to untreated controls in both S. aureus ATCC ® 25923 type strain and USA300.

Techniques: Western Blot, Expressing, Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: Nusbiarylins Inhibit Transcription and Target Virulence Factors in Bacterial Pathogen Staphylococcus aureus

doi: 10.3390/ijms21165772

Figure Lengend Snippet: Effects of selected nusbiarylins on α-toxin-mediated lysis of rabbit red blood cells (RBCs) in ( A ) S. aureus ATCC ® 25923 and ( B ) MRSA USA300 (readings normalised with maximum as 100%) at subinhibitory concentrations, shown as ( i ) plate image and ( ii ) % lysis plot derived from absorbance changes. Supernatants from both drug-treated and untreated overnight bacterial cultures were serially diluted (× = folds diluted) and incubated with suspension of packed rabbit RBCs, with plain phosphate-buffered saline (PBS) as negative lysis control and 1% Triton X-100 as positive lysis control. Experiments were repeated in duplicates and representative data presented. Van: vancomycin; Rif: rifampicin.

Article Snippet: Vancomycin had no apparent effect on α-toxin production compared to untreated controls in both S. aureus ATCC ® 25923 type strain and USA300.

Techniques: Lysis, Derivative Assay, Incubation, Suspension, Saline, Control

Journal: International Journal of Molecular Sciences

Article Title: Nusbiarylins Inhibit Transcription and Target Virulence Factors in Bacterial Pathogen Staphylococcus aureus

doi: 10.3390/ijms21165772

Figure Lengend Snippet: Distribution of C T values of some commonly used “housekeeping” genes. Relative qPCR assay signal levels of ( A ) 16S rRNA, ( B ) 23S rRNA, ( C ) gyrA , ( D ) gyrB and ( E ) gmk in S. aureus ATCC ® 25923, 29213 and MRSA strain USA300. Data irrespective of strains or treatment groups were all plotted together as y -values and segregated by their corresponding replicate sets on the x -axis. Experiments were performed in replicates.

Article Snippet: Vancomycin had no apparent effect on α-toxin production compared to untreated controls in both S. aureus ATCC ® 25923 type strain and USA300.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Nusbiarylins Inhibit Transcription and Target Virulence Factors in Bacterial Pathogen Staphylococcus aureus

doi: 10.3390/ijms21165772

Figure Lengend Snippet: Effects of subinhibitory levels of MC4 and rifampicin on the relative expression of virulence-associated genes in ( A ) S. aureus ATCC ® 25923 and ( B ) MRSA strain USA300. Probed transcripts belong to the staphylococcal SarA/SarR ( sarA ) and agr regulons ( agrA , RNAII, RNAIII), as well as clinically significant exotoxins Protein A ( spa ), α-toxin ( hla ) and PVL ( lukS ). Fold change values (2 − ΔΔ CT for upregulation; 1/−2 − ΔΔ CT for downregulation) were extrapolated from ΔΔC T calculations. Experiments were performed in triplicates. 25923: S. aureus ATCC ® 25923; USA300: CA-MRSA strain USA300; Rif: rifampicin.

Article Snippet: Vancomycin had no apparent effect on α-toxin production compared to untreated controls in both S. aureus ATCC ® 25923 type strain and USA300.

Techniques: Expressing